Novosphingobium sediminis sp. nov., isolated from the sediment of a eutrophic lake

Abstract

A Gram-negative, rod-shaped, aerobic, non-motile, bacterium (YG-17T) was isolated from eutrophic Taihu lake sediment. Colonies grown on YG agar plates were circular, convex, and yellowcolored. On the basis of 16S rRNA gene sequence similarity, strain YG-17T was shown to be closely related to Novosphingobium aromaticivorum (97.9%), followed by Novosphingobium stygium (97.5%), Novosphingobium subterranea (96.9%) and Novosphingobium taihuense (96.7%). The DNA-DNA relatedness values of strain YG-17T to the most phylogenetically related species N. aromaticivorum and N. stygium were 14% and 21%, respectively. The G+C content of the genomic DNA was 63.2 mol%. The major respiratory quinone was ubiquinone-10 and major fatty acids were C18:1ω7c, C17:1ω6c and C14:02-OH. Sphingoglycolipids were present, and spermidine was detected as the major polyamine. According to comparative physiological and chemotaxonomic analysis, strain YG-17T is proposed to be a novel species of the genus Novosphingobium, with the name of Novosphingobium sediminis sp. nov. The type strain is YG-17T (NBRC 106119T= CGMCC 1.9114T).