Efeitos do exercício agudo sobre biomarcadores séricos de ratos diabéticos

Abstract

Introduction: The responses to acute exercise on biomarkers, such as alkaline phosphatase (ALP) and creatine kinase (CK) serum levels have been little investigated in diabetic rats. Objectives: To investigate the effects of acute aerobic exercise on the concentrations of CK and ALP as well as evaluating the hydration status in diabetic rats. Materials and methods: Were used male Wistar rats, young adults, divided into two groups: diabetic (DA) and controls (CA). The diabetes was induced in the rats by administration of alloxan monohydrate Sigma® (32 mg/kg body weight). Two weeks after confirmation of diabetes, both groups were subjected to an acute swim session for 30 min, with aerobic load (4.5% body weight). Glucose, hematocrit, CK, ALP, albumin and lactate kinetics during exercise were evaluated by collecting 25μL of blood from the tail of the animals in minutes 0, 10, 20 and 30 of exercise. Results: Two-way ANOVA for repeated measures and post hoc Tukey test showed significant decrease of glycemia after exercise for the DA group, significant increase in CK after exercise for the DA group, significant increase in hematocrit for both groups after exercise and maintenance of ALP after exercise for the DA group. Conclusion: The acute aerobic exercise was effective in controlling glucose levels in diabetic rats. However, it should be applied with caution, because it induced high CK values, suggesting possible tissue damage.