Concentration of Exopolysaccharides (EPS) Produced from Fermentation of Hydrolysate Biomass of Tuni Sagoo (Metroxylon sp.) Starch by Lactobacillus plantarum FU-0811 in Semi Pilot Scale using Nanofiltration

Abstract

Nanofiltration (NF) system is one of the concentration process step to recover exopolysaccharides (EPS) concentrate (retentate) as food aditive (thickener, stabilizer and emultion). Feed was biomass containing EPS as a result of growth of Lactobacillus plantarum FU-0811 culture under 37 C for 72 hours in semi pilot scale (20 L). Biomass was a growth media containing sagoo sugar as a result of step enzymatic hydrolysis on tuni sagoo (Metroxylon sp.) starch using commercial α-Amylase and Amyloglucosidase (AMG) enzymes and other active compounds. Concentration process was conducted by pump motor frequency of 20 Hz, room temperature, and pressure of 10, 15 and 20 bar for 0, 30, 60, 90 and 120 minutes. Result of experimental work showed that NF system has potential use to concentrate biomass containing higher composition than that permeate. Based on optimum EPS, NF system that results a successfully separation of EPS with rejection of 95.24 % was reached at pressure of 20 bar and 60 minutes to retain EPS in concentrate (retentate) of 2.454 % (dry weight) and freely pass in permeate of 0.0097 % (dry weight) with permeate flux value 37.5 L/m2.hour. In this optimum process condition, NF system was able to increase of EPS in retentate of 56.46 % from prior to process (feed) and after process concentration.