Wound healing activity of chrysophyllum cainito L. Leaves: Evaluation in rats using excision wound model

Abstract

Background: The present study was aimed to evaluate the traditionally reported wound healing potential of Chrysophyllum cainito leaves. Methods: The ethanolic extract of C. cainito leaves was standardized in terms of its gallic acid content (a phenolic compound with wound healing properties) using a validated HPTLC technique. The antioxidant activity of C. cainito leaves was also evaluated in vitro. The wound healing potential of the extract was evaluated in male Albino Wistar rats using excision wound model. The results were statistically evaluated and compared with traditional drug Jatyadi Taila (JT) and modern drug Betadine (BTD). Results: Topical application of the standardized ethanolic extract of C. cainito leaves on excision wounds caused the significantly faster reduction in the wound area as compared to JT and BTD. Similarly, it also showed the significant increase in the tissue biochemical parameters such as hydroxyproline, hexosamine and protein when compared with the untreated control. Conclusion: Findings of the present study provide a baseline data on wound healing and antioxidant potential of C. cainito leaves and supports their traditional claim.