BRINE SHRIMP LETHALITY BIOASSAY OF ABRUS PRECATORIUS (LINN) LEAVES AND ROOT EXTRACT

  • Y. W
  • R. P
  • A. F
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Abstract

<p><strong>Objective: </strong>The present study was conducted to test for <em>in vivo</em> Brine Shrimp Lethality Assay (BSLA) of <em>Abrus precarious</em><em> </em>leaves and root extracts after successive maceration in four solvents (n-hexane, dichloromethane (DCM) ethyl acetate and methanol) and correlate cytotoxicity results with known pharmacological activities of the plant.</p><p><strong>Methods: </strong>Cytotoxicity was evaluated in terms of LC<sub>50</sub> (lethality concentration), 10 nauplii were added into three replicates of each concentration of the plant extracts, and after 24 h the surviving brine shrimp larvae were counted, and LC<sub>50</sub> was assessed.</p><p><strong>Results: </strong>Potent cytotoxicity was found for both the leaves and root extracts of <em>Abrus precatorius</em>, results showed a concentration dependent increment in mortality rate of the brine shrimp nauplii and the n-hexane and dichloromethane fractions of the root and leaves extracts were more potent against the brine shrimp with LC<sub>50</sub> values of 7.870 ppm and 19.135 ppm (µg/ml) respectively, whereas methanol fractions of both the extracts exhibited low potent activity with LC<sub>50</sub> values 61.575 ppm and 226.053 ppm (µg/ml) in root and leaves respectively.</p><p><strong>Conclusion: </strong>The result indicated bioactive components are present in this plant that could be accounted for its pharmacological effects.</p>

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Y., W. H., R., P. D., & A., F. B. (2016). BRINE SHRIMP LETHALITY BIOASSAY OF ABRUS PRECATORIUS (LINN) LEAVES AND ROOT EXTRACT. International Journal of Pharmacy and Pharmaceutical Sciences, 9(1), 179. https://doi.org/10.22159/ijpps.2017v9i1.15057

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