Estimation of bacoside-A in Bacopa monnieri aerial parts using TLC densitometry

Abstract

Objective: To estimate bacoside A in Bacopa monnieri aerial parts using a validated TLC densitometric method. Methods: The content of bacoside A was estimated using standardized procedures. A standard curve was prepared by applying different concentrations of the marker. The estimation of bacoside A was achieved on precoated TLC plate using solvent system, i.e., toluene: ethyl acetate: methanol: formic acid (3:3.5:2.5:1) followed by scanning at 225 nm. The developed method was validated for the parameters described in the ICH guidelines. Results: The content of bacoside A was determined to be 1.13±0.019% w/w in B. monnieri aerial parts. The instrumental precision, repeatability, linearity range, correlation coefficient, intra-day precision, inter-day precision, limit of detection (LOD), limit of quantification (LOQ) and accuracy were found to be 0.61% CV, 0.87% CV, 150-900 ng, 0.998, 1.43% CV, 1.79% CV, 32 ng/spot, 92 ng/spot and 97.19±0.39% respectively. Conclusion: The developed method was found to be simple, economic, accurate, precise, and can be utilized for the quantitative as well as qualitative estimation of bacoside A in Bacopa monnieri.