Morfologia e quantificação da microbiota intestinal do curimbatá (Prochilodus lineatus) e do cascudo cinza (Pterygoplichthys anisitsi) cultivados em cativeiro

Abstract

All animals exist in intimate associations with microorganisms that play important roles in the hosts' normal development. In vertebrates, the most populous and complex community of microbes resides in the digestive tract. The aim of this research was to morphologically quantify, classify and verify the composition of intestinal microbiota of two species of freshwater fish, the Prochilodus lineatus and the Pterygoplichthys anisitsi. The samples were collected by scrapings of intestinal mucosa, serially diluted to 10-4, plated on tryptic soy agar (TSA) and chocolate agar (CA) for total bacterial counting and morphological identification by Gram, in aerobiosis and facultative anaerobiosis conditions, respectively. In the total bacterial counting results ranged between 103 to 104 cfu.mL-1. The morphological types found were cocci, yeasts and Gram negative and positive rods. Additional studies about patterns of microbial colonization and the morphology of the adhered microorganisms to the intestinal mucosa were possible using the scanning electron microscopy (SEM) and several forms of microorganisms, such as yeasts, cocci and bacillary shapes flagellated and non-flagellated were found. The intestinal microbiota of P. lineatus and P. anisitsi was diverse and populous, with a predominance of Gram negative microorganisms.