Systemic κAL Amyloidosis Associated with Bovine Leukocyte Adhesion Deficiency

Abstract

Histopathologic and immunohistochemical examinations were conducted on a 5-year-old Holstein-Friesian cow with systemic κAL amyloidosis associated with bovine leukocyte adhesion deficiency. Amyloid deposits were present in the perivascular and intercellular spaces of the visceral organs, such as the liver, kidneys, pancreas, adrenal glands, and upper alimentary tract. Amyloid was stained positively with Congo red with or without 5% potassium permanganate pretreatment and had green birefringence observed under polarized light. Immunohistochemically, amyloid reacted strongly against anti-bovine IgG (H+L) and anti-bovine κ-light chain and reacted weakly against bovine λ-light chain antibodies but was negative for anti-human amyloid AA antibody. This is the first description of AL amyloidosis immunohistochemically related to immunoglobulin κ-light chains of precursor protein in cattle.