Antiatherosclerotic potential of active principle isolated from Eugenia jambolana in streptozotocin-induced diabetic rats

Abstract

The aim of the present study was to investigate the antiatherosclerotic effect of active principle (FIIc) isolated from aqueous fruit pulp extract of Eugenia jambolana. Crude aqueous extract of E. jambolana was subjected to purification using chromatographic techniques which yielded purified active compound (FIIc). Purity of FIIc was tested by HPLC. Phytochemical investigation of FIIc by NMR, IR, and UV spectra showed that the purified compound is -hydroxy succinamic acid. The streptozotocin- (STZ-) induced diabetic rats were fed atherosclerotic (Ath) diet containing 1.5mL olive oil containing 8mg (3, 20,000 IU) vitamin D 2 and 40mg cholesterol for 5 consecutive days. The STZ-induced diabetic rats receiving Ath diet were orally administered FIIc at doses of 10, 15, and 20mg/kg, and results were compared with reference drug, that is, glibenclamide (600 g/mg) and healthy control. 30-day treatment with FIIc resulted in significant (P