Effects of waterborne uranium on survival, growth, reproduction and physiological processes of the freshwater cladoceran Daphnia magna

Abstract

Acute uranium toxicity (48 h immobilisation test) for Daphnia magna was determined in two different exposure media, differing in pH and alkalinity. LC50 varied strongly between media, from 390 ± 40 μg L-1 U at pH 7 to 7.8 ± 3.2 mg L-1 U at pH 8. According to the free ion activity model uranium toxicity varies as a function of free uranyl concentration. This assumption was examined by calculating uranium speciation in our water conditions and in those reported in the literature. Predicted changes in free uranyl concentration could not solely explain observed differences in toxicity, which might be due to a competition or a non-competitive inhibition of H+ for uranium transport and/or the involvement of other bioavailable chemical species of uranium. Chronic effects of uranium at pH 7 on mortality, ingestion and respiration, fecundity and dry mass of females, eggs and neonates were investigated during 21-day exposure experiments. A mortality of 10% was observed at 100 μg L-1 U and EC10 for reproduction was 14 ± 7 μg L-1 U. Scope for growth was affected through a reduction in feeding activity and an increase in oxygen consumption at 25 μg L-1 U after 7 days of exposure. This had strong consequences for somatic growth and reproduction, which decreased, respectively, by 50% and 65% at 50 μg L-1 U after 7 days and at 25 μg L-1 U after 21 days. Uranium bioaccumulation was quantified and associated internal alpha dose rates from 2.1 to 13 μGy h-1 were estimated. Compared to the toxicity of other alpha-emitting radionuclides and stable trace metals, our results confirmed the general assumption that uranium chemical toxicity predominates over its radiotoxicity. © 2007 Elsevier B.V. All rights reserved.