Characterization of the raptor/4E-BP1 interaction by chemical cross-linking coupled with mass spectrometry analysis

Abstract

Background: mTORC1 recruits its substrate 4E-BP1 via Raptor/4E-BP1 interaction. Chemical cross-linking/mass spectrometry permits characterization of protein-protein interactions. Results: Cross-linked peptides between Raptor and 4E-BP1 were identified. Raptor intramolecular cross-links were also identified. Conclusion: Raptor N-terminal region containing RNC1 is implicated in the interaction with the central region of 4E-BP1. Significance: Our study provides novel insight into how mTORC1 recognizes 4E-BP1. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.