Natural killer (NK) cells were shown to secrete differentially interleukins (IL), IL-1α, IL-1β, IL-2, IL-8, interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), and leukaemia inhibitory factor (LIF) upon stimulation with optimal concentrations of chylomicrons (CM), very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL) or acetyl-modified low-density lipoprotein (AcLDL). CM, VLDL, LDL and AcLDL induced LIF secretion which was absent in non-stimulated cells. CM, VLDL, and LDL did not affect IL-1α secretion. CM stimulated IL-8 > TNF-α > IL-1α > IL-2 = IFN-γ, and decreased seventeenfold GM-CSF secretion. VLDL stimulated IL-8 secretion > IL-1α = IL-2 > IFN-γ > TNF-α and decreased fivefold GM-CSF secretion. LDL stimulated IL-8 secretion > IL-1α > IL-2 = IFN-γ, it did not modify TNF-α and inhibited five hundredfold GM-CSF secretion. HDL stimulated IL-2 secretion = IFN-γ > IL-8, it decreased GM-CSF secretion > IL-1α > IL-1β > TNF-α without affecting LIF. AcLDL stimulated IL-8 secretion > TNF-α > IL-1α > IL-2 = IFN-γ = IL-1β, and decreased GM-CSF secretion eightfold. When NK cells were primed with 10, 100 or 500 IU/ml of IL-2 before the addition of lipoproteins, a decrease in the secretion of cytokines was observed as compared with cells primed with IL-2 only. Differences in cytokine secretion were observed among the diverse type of lipoproteins used for cell stimulus. Thus, lipoproteins may condition NK cytokine secretion and cell activation.
CITATION STYLE
De Sanctis, J. B., Blanca, I., & Bianco, N. E. (1997). Secretion of cytokines by natural killer cells primed with interleukin-2 and stimulated with different lipoproteins. Immunology, 90(4), 526–533. https://doi.org/10.1046/j.1365-2567.1997.00174.x
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