Viability Spermatozoa Epididymis of Buffalo (Bubalus Bubalis) in Fertilized Media to Additional Serum at Temperature 5°C

Abstract

This study purposed to determine the quality of Buffalo's epididymis Spermatozoa on the TALP (Tyrode Albumin Sodium Lactate Sodium Pyruvate) fertilization at the 5°C temperature. The Randomized Block Design used on this research with 3 treatments and 5 blocks as replication. The treatments were additional serum: P1 (0%), P2 (10%) and P3 (20%). The results showed that the highest motility was on the treatment P3 (20%), about 66.00±9.45 was significantly effect (P<0.05) than P1 and P2. The highest percentage of live on P3 (20%) was 75.20±2.41 and significantly effect (P<0.05) than P1 and P2. The best percentage of abnormality was also on the P3 (20%) i.e.14.20±1.30 significantly effect (P<0.05) compare P1 and P2. It could be concluded that the addition of 20% serum in TALP media as a fertilization medium can improve the buffalo's spermatozoa quality of caudal epididymis.