Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells

Abstract

The human rotavirus titer was optimal at an infection cell density of about 4-8 x 104 cells cm-2 in monkey kidney cell cultures. The highest viral titers (3.8 x 107 TCID50 ml-1 and 3.7 x 107 TCID50 ml-1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.