Immobilization of trypsin enzyme on silver nanoparticles

Abstract

Introduction and Aim: Immobilization of enzyme on silver nanoparticles (AgNPs) is one way to improve their stability and activity and can be reused for large-scale applications. The present study was aimed to evaluate and characterize the trypsin enzyme immobilized on biogenically synthesized silver nanoparticles. Materials and Methods: Immobilization of trypsin enzyme was optimized with time and varying concentration of silver nanoparticles, which were green synthesized using avocado seed extracts. The residual activity of trypsin enzyme after immobilization was estimated. The reusability and temperature stability of the immobilized enzyme were studied. Results: The immobilized enzyme showed maximum activity of 1006.0 U during the time duration of 18 hr of incubation with AgNPs. Reusability of immobilized enzyme in avocado AgNPs was assessed under optimal conditions, the activity of immobilized enzyme was loss after 5 repeated cycles. The enzyme captured on AgNPs was released in the presence of anionic detergent. Temperature stability was assessed for immobilized enzyme on AgNPs and the immobilized enzyme was stable up to 55ºC and later it loss their activity. Conclusion: The study reports a feasible method of enzyme immobilization on biogenically synthesized silver nanoparticles. The enzyme immobilized on silver nanoparticles is mainly through non-covalent forces. Further studies are required to improve the reusability of enzyme.