Bioactive Derivatives of Curcumin Attenuate Cataract Formation In Vitro

Abstract

In this study, curcumin derivatives salicylidenecurcumin (CD1) and benzalidenecurcumin (CD2)] were prepared, and their biological activity was compared in in vitro selenite-induced cataract model. The antioxidant activity was studied using DPPH radical scavenging assay. Knoevenagel condensates of curcumin exhibited higher DPPH radical scavenging activity compared with curcumin. The anticataractogenic potential of curcumin derivatives was analyzed using lens organ culture method. The activity of antioxidant enzymes and calcium homeostasis was reversed to near normal levels following treatment in organ cultured rat lenses. These results indicated that curcumin and its derivatives-CD1 and CD2-are beneficial against selenite-induced cataract in vitro. Of these, CD1 is having higher bioactive potential compared with curcumin and CD2. The salicylidenecurcumin (CD1) has an additional OH in the salicylidene, resulting in an appreciable increase in its antioxidant property. Thus the mere increase of phenolic OH group will enhance the antioxidant property. Here CD1 showed higher antioxidant acitivity than curcumin and CD2. © 2012 John Wiley & Sons A/S.