Wastes of agroindustry an alternative to develop biopreparates with probiotic capacity

Abstract

The objective of this study was to obtain and characterize three candidate microbial cultures for veterinary probiotics, developed in different substrates on agro-industry wastes. A selection of lactic acid bacteria (Lactobacillus acidophilus, L. bulgaricus, Streptoccus thermophillus) and yeasts of Kluyveromyces fragilis (L-4 UCLV) were used to develop probiotic preparations. The substrates chosen were: Molasses (as a source of carbohydrates) and soy milk, whey, torula yeast and orange vinasse (as a source of protein) to obtain a high number of microorganisms and levels of organic acids. The substrates under study were composed of: T1, molasses and soy milk. T2, buttermilk more torula yeast and molasses. T3, orange vinasse and molasses. All the variables were incubated for 24 at 37°C. Dry matter, crude and true protein, ether extract, ash, viability and microbial concentration were determined in all treatments (T1, T2 and T3). At the time of incubation, the pH values of all the treatments were lower than 4.22 and at 24 hours it was reduced to 3.86. The values of the dry material and ether extract were higher (P<0.05) in T1; the crude and true protein was higher (P<0.05) in T2; whereas, ash was higher (P<0.05) in T3. But for all the variables the microbial concentration and the viability was higher than 93% and 7.7x107 cfu/mL respectively. The results of the present study showed that byproducts such as: Molasses, soy milk, whey, milk yeast and orange vinasse are suitable substrates to develop microorganisms with probiotic capacity and obtain an acceptable probiotic for veterinary use.