Effect of Applied Voltages on Corn Stover Biomethanation and Microbial Community Characteristics in a Microbial Electrolytic Cell-Assisted Anaerobic Digestion System

Abstract

This study aims to investigate the effect of different applied voltages on the biomethanation performance and microbial community characteristics of corn stover (CS) in a microbial electrolysis cell (MEC)-assisted anaerobic digestion (AD) system (MEC-AD). The results showed that the MEC-AD system operating at 0.8 V achieved the highest methane yield of 192.40 mL CH4/g VS (volatile solids), an increase of 14.98% compared to the conventional AD. The system obtained methane yields of 187.74 to 191.18 mL CH4/g VS at lower voltages (0.4 V and 0.6 V), and 156.11–182.75 mL CH4/g VS at higher voltages (1.0 V and 1.2 V), respectively, suggesting that lower or higher voltages would have adversely impacted the methane yield. Correspondingly, the MEC-AD system operating at 0.4–0.8 V achieved over 71.47% conversion rates of total solids (TS), VS, and cellulose. The microbial community analysis revealed that 0.8 V optimally enriched fermentative acidogenic bacteria (FABs, 24.55%) and electroactive bacteria (13.50%), enhancing both hydrolysis acidification efficiency and direct interspecies electron transfer (DIET). Both Methanosarcina and Methanoculleus demonstrated significant positive correlations with FABs, SOBs, and electroactive bacteria. This study reveals that 0.8 V represents the optimal operating voltage for biomethane production in MEC-AD systems, providing critical insights for agricultural waste valorization.