miR-34a increases inflammation and oxidative stress levels in patients with necrotizing enterocolitis by downregulating SIRT1 expression

Abstract

The miR-34a/SIR T1 signaling axis is an important signaling axis in tumors and diseases. Notably, low SIR T1 expression in the intestinal tissues of patients with necrotizing enterocolitis (NEC ) has been reported. However, whether miR-34a/SIR T1 signaling as a target to protect the intestines during the NEC process is unclear and remains to be elucidated. Blood samples were collected from 30 patients with NEC , and an NEC rat model was used. The miR-34a and SIR T1 gene and protein expression levels were assayed by qPCR and Western blotting method. The inflammatory cytokine levels and oxidative stress levels were detected using the ELI SA method. The results demonstrated that birth weight, albumin and glucose concentrations were significantly decreased in the NEC patient group compared with the control group, but the C-reactive protein (CR P) and procalcitonin (PCT) concentrations were significantly increased. The miR-34a expression level was notably increased in the NEC group, but the SIR T1 expression level was markedly decreased. Notably, the miR-34a was significantly correlated with NEC severity and the concentrations of CR P, PCT, IL -6, TNF-α, IL -1β, IL -8, MCP-1, VCA M1 and malondialdehyde (MDA), but was significantly negatively correlated with SIR T1 gene expression and the concentration of IL -10. Intestinal villi damage in NEC rats was decreased with miR-34a inhibition and SIR T1 activation treatment by decreasing the levels of inflammatory cytokines, including IL -6, TNF-α, IL -1β and IL -8, and oxidative stress proteins, including MCP-1, VCA M1, and MDA , as well as increasing the level of the anti-inflammatory cytokine IL-10. In addition, the results indicated that miR-34a inhibition and SIR T1 activation strongly protected the intestine and decreased the damage caused by NEC , not only by decreasing the protein levels of SIR T1, TNF-α, IL -1β, IL -6 and IL -8, but also by increasing the IL -10 protein levels. The miR-34a inhibition and SIR T1 activation may decrease the damage caused by NEC by decreasing proinflammatory cytokines and oxidative stress proteins and by increasing the anti-inflammatory cytokine pathway. Based on the aforementioned analysis, the miR-34a and SIR T1 proteins may be potential novel therapeutic targets in NEC .