A v-SNARE implicated in intra-golgi transport

Abstract

We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, a Golgi SNARE of 28 kD, is efficiently packaged into Golgi- derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind α-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP- coated vesicles, but do accumulate docked uncoated vesicles.