Advanced phytochemistry and chemo-metric profiling of the bioactive medicinal components of n-hexane seed extract of Xylopia aethiopica using FTIR and GC-MS techniques

Abstract

The use of herbal medicine has achieved global success as an ethno-medicine, yet there are lots of drawbacks in their commercial value and wide acceptance due to poor regulation and standardization. Present study aims to chemo-metrically characterize the phyto-chemical principles present in the n-hexane seed extracts of Xylopia aethiopica using chromatographic and spectroscopic methods. The crude n-hexane extracts were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) model 8400S and GC-MS (Model-QP 2010 plus Spec). The identification of compounds was done using NIST ver.2.0-year 2005 Library. The biological activity are based on Dr. Duke’s phytochemical and ethno-botanical databases. The FTIR revealed functional groups such as alkenes, alkanes, alcohols, aromatic rings as well as fatty acids. The GC-MS revealed phyto-compounds such as alpha-Terpineol, alpha-cubabene, pinocarvone, copaene and alpha-muurolene. The presence of these vital phytochemicals with excellent pharmacological activity may explain their usage in traditional medicine.