miR-433 protects pancreatic β cell growth in high-glucose conditions

Abstract

Pancreatic β cell dysfunction is a key characteristic in the pathogenesis of diabetes mellitus (DM). MicroRNAs (miRNAs) have been identified to serve a role in DM pathogenesis, but how specific miRNAs regulate glucose-stimulated β cell functions remain unclear. The present study aimed to explore the effects of miR-433 on cell growth under high-glucose culture conditions and to determine the possible mechanisms involved. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the expression levels of miRNAs in Min-6 pancreatic β cells cultured in high-glucose medium, which revealed that miR-433 was significantly downregulated. Results from in vitro Cell Counting Kit-8, colony formation and flow cytometry analyses indicated that overexpression of miR-433 may enhance cell viability and proliferation by promoting cell cycle progression and suppressing apoptosis. Furthermore, bioinformatics prediction and luciferase analysis demonstrated that miR-433 was able to inhibit the expression of cyclooxygenase 2 (COX2) through targeting its 3'-UTR. Moreover, knockdown of COX2 expression alleviated the inhibition of cell growth induced by high glucose, similar to overexpression of miR-433. In conclusion, the present results suggested that miR-433 may protect pancreatic β cells cultured in high glucose, which suggests that miR-433 may have beneficial effects in preventing and treating DM.