Biosynthesis of silver nanoparticles using Ulva fasciata (Delile) ethyl acetate extract and its activity against Xanthomonas campestris pv. malvacearum

Abstract

Metallic nanoparticles have been traditionally synthesized using wet chemical techniques, where the chemicals used are quite often toxic and flammable. In this research, we present a simple and ecofriendly biosynthesis of Ag nanoparticles using Ulva fasciata crude ethyl acetate extract as reducing and capping agent. The bionanoparticle characterized with UV-vis Spectroscopy, FTIR, XRD, SEM and EDX. Characterization reveals that the nanoparticles are crystalline in nature, spherical in shape and poly-dispersed with size ranging from 28 to 41 nm. The alkyne group (3424.30 cm -1) of U. fasciata crude ethyl acetate extract shifted and reduced the AgNO 3. GC-MS analysis revealed the presence of 1-(Hydroxymethyl)-2, 5, 5, 8A-tetramethyl decahydro-2- napthalenol as reducing agent and hexadecanoic acid was found to be a stabilizing agent. Ulva fasciata based bionanoparticles inhibited the growth of Xanthomonas campestris pv. malvacearum (14.00±0.58 mm Zone of inhibition), with a Minimum Inhibitory Concentration of 40.00±5.77μg/mL. The study shows that U. fasciata crude ethyl acetate extract could be used as a reducing agent for simple ecofriendly synthesis of silver nanoparticles. However, more studies are essential before recommending them for disease management. © JBiopest.