Construction and Use of an Inexpensive in Vitro Ultrasonic Misting System

Abstract

An inexpensive user-constructed ultrasonic misting system to grow plant tissues in vitro is presented. This system is constructed by coupling two commercially available products, a culture chamber and an ultrasonic humidifier. Plant cultures are grown within a culture chamber on a platform and bathed periodically by an ultrasonic nutrient mist. Carrot cultures were found to grow as much as four to 10 times as great as those grown on agar medium.