Beacon-mediated exponential amplification reaction (BEAR) using a single enzyme and primer

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Abstract

Beacon-mediated Exponential Amplification Reaction (BEAR) enables isothermal, exponential signal amplification. BEAR uses only a single enzyme and a single primer. Detection of 0.2 amol of a mitochondrial DNA with a point mutation in less than an hour demonstrates an application of the BEAR technique for nucleic acid research.

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Newbigging, A. M., Zhang, H., & Le, X. C. (2019). Beacon-mediated exponential amplification reaction (BEAR) using a single enzyme and primer. Chemical Communications, 55(72), 10677–10680. https://doi.org/10.1039/c9cc04226a

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