Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats

39Citations
Citations of this article
46Readers
Mendeley users who have this article in their library.

Abstract

Peptide gH625, derived from glycoprotein H of herpes simplex virus type 1, can enter cells efficiently and deliver a cargo. Nanoparticles armed with gH625 are able to cross an in vitro model of the blood–brain barrier (BBB). In the present study, in vitro experiments were performed to investigate whether gH625 can enter and accumulate in neuron and astrocyte cell lines. The ability of gH625 to cross the BBB in vivo was also evaluated. gH625 was administered in vivo to rats and its presence in the liver and in the brain was detected. Within 3.5 hours of intravenous administration, gH625 can be found beyond the BBB in proximity to cell neurites. gH625 has no toxic effects in vivo, since it does not affect the maximal oxidative capacity of the brain or the mitochondrial respiration rate. Our data suggest that gH625, with its ability to cross the BBB, represents a novel nanocarrier system for drug delivery to the central nervous system. These results open up new possibilities for direct delivery of drugs into patients in the field of theranostics and might address the treatment of several human diseases.

Cite

CITATION STYLE

APA

Valiante, S., Falanga, A., Cigliano, L., Iachetta, G., Busiello, R. A., La Marca, V., … Galdiero, S. (2015). Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats. International Journal of Nanomedicine, 10, 1885–1898. https://doi.org/10.2147/IJN.S77734

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free