A novel phospholipase A 2 (PLA 2) gene, named PfPLA 6, was found in a 6,328-bp NIS-1(5′)-a segment in the Protobothrops flavoviridis (Habu, Crotalinae) genome. A comparison of the aligned nucleotide sequences of Viperidae (Viperinae and Crotalinae) venom PLA 2 genes, including PfPLA 6, revealed the deletion of a 12-bp segment called S1EX 1 and a 55-bp segment called S2EX 1 in exon 1 and the interposition of a 219-bp segment called SINT 2 (SINE) in intron 2. A classification of Viperidae PLA 2 genes based on these structural modes indicated that the A-type genes (without SINE), including PfPLA 6, are evolutionarily ancestral to the Btype (Viperinae) and C-type (Crotalinae) PLA 2 genes (both with SINE). Since PfPLA 6 is a pseudogene, an active prototype of PfPLA 6 can be assumed to be the ancestral PLA 2 gene. Putative evolutionary processes from this A-type prototype PLA 2 gene to descendent PLA 2 genes are discussed.
CITATION STYLE
Chijiwa, T., Ikeda, N., Masuda, H., Hara, H., Oda-Ueda, N., Hattori, S., & Ohno, M. (2012). Structural characteristics and evolution of a novel venom phospholipase A 2 gene from protobothrops flavoviridis. Bioscience, Biotechnology and Biochemistry, 76(3), 551–558. https://doi.org/10.1271/bbb.110848
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