Molecular basis of genetic diversity among cytoplasms of triticum and aegilops species. Iv. ctDNA variation in ae. triuncialis

Abstract

Restriction endonuclease analysis of chloroplast DNA of Ae. triuncialis was carried out. Thirteen accessions had the type 2a chloroplast genome derived from Ae. caudata, eight possessed type 3 chloroplast genome of Ae. umbellulata, and the remaining five contained a new chloroplast genome (named type 2b) differing from the former two, by a 0·3 kbp insertion and four base substitutions, respectively. The accessions with type 2a and type 3 chloroplast genomes distribute in wide areas, and in both of its subspecies, eu-triuncialis and orientalis, whereas those having the type 2b chloroplast genome occur only locally in Azerbaijan, Transcaucasus. From these results, the following two conclusions are drawn; (a) Ae. triuncialis originated from the reciprocal crosses between Ae. caudata and Ae. umbellulata, and (b) the type 2b chloroplast genome was arisen from type 2a chloroplast genome by a 0·3 kbp insertion. © 1986 The Genetical Society of Great Britain.