Identification of Contamination in the American Type Culture Collection Stock of Human Adenovirus Type 8 by Whole-Genome Sequencing

Abstract

Here we report on the whole-genome sequences and phyloge- netic analyses ofthe HAdV-8 and HAdV-10 reference virus stocks distributed by the ATCC and conclude that HAdV-8 lot number 59323195, provided by the ATCC, contained both HAdV-8 and HAdV-10 types. Due to HAdV-8 being one ofthe major causative agents ofEKC, we would like to draw attention ofthe adenovirus research community to this contamination issue so as to prevent misinterpretation. Nucleotide sequence accession numbers. The whole-genome sequences of the HAdV-8 Trim variant, the HAdV-10 contami- nant in the ATCC HAdV-8 stock, HAdV-10 NIID-1, and the HAdV-10 prototype have been deposited at DDBJ under acces- sion numbers AB746853, AB746854, AB695621, and AB724351, respectively. ACKNOWLEDGMENTS