High Bacterial Cellulose Production by Acetobacter xylinum ATCC 10245 in a New Culture Medium with a Sulfite Pulping Waste Fraction

Abstract

Higher cellulose production was observed in a new culture medium (named KC medium) consisting of a sulfite pulping waste fraction (named CP powder), glucose, yeast extract, peptone and buffer components as compared with the standard 2% glucose Hestrin-Schramm culture medium (HS medium). Acetobacter xylinum ATCC 10245 cultivated in KC meduim of 2% glucose gave high cellulose yields (296%), and the highest yield was obtained at the 3% dosage of glucose and 0.5% dosage of the CP powder (326%). The fractionation and the ion exchange experiments suggested that the high molecular lignosulfonate contributed to the yield enhancement. © 1994, The Society of Fiber Science and Technology, Japan. All right reserved.