Positive inotropic and lusitropic effects mediated via the low-affinity state of β 1-adrenoceptors in pithed rats

Abstract

1. Activation by CGP 12177 and cyanopindolol of the human and rat low-affinity state of β 1-adrenoceptors increases frequency and contractile force and hastens relaxation in isolated cardiac tissues, and probably relaxes isolated vessels. In order to identify the positive inotropic, positive lusitropic and vasodilator effects of both agonists also in vivo, we have determined their effects on the left ventricular systolic pressure (LVSP), the rate of intraventricular pressure rise (+dPdt max-1) and decline (-dPdt max-1), the diastolic blood pressure (DBP) and the mesenteric blood flow (MBF) in pithed and vagotomized rats. 2. CGP 12177 (0.1-100 nmol kg -1) and cyanopindolol (1-1000 nmol kg -1) dose-dependently enhanced all cardiac parameters. The nonselective β-adrenoceptor antagonist bupranolol 10 μmol kg -1 diminished the CGP 12177 (100 nmol kg -1)-stimulated increases in LVSP from 26.3±8.2 to 13.1±1.8 mmHg (P<0.05), +dPdt max-1 from 5287±290 to 2439±296 mmHg s -1 (P<0.001) and -dPdt max-1 from -3836±301 to -2187±443 mmHg s -1 (P<0.05), respectively. The β 1-adrenoceptor antagonist CGP 20712A 10 μmol kg -1 (known to block the low-affinity state of β 1-adrenoceptors at high doses) inhibited increases in ±dPdt max-1 elicited by the highest dose of CGP 12177. 3. The highest doses of CGP 12177 and cyanopindolol increased DBP by about 10 mmHg and MBF by 1.4±0.3 and 0.6±0.3±ml min -1, respectively. The vascular effects of CGP 12177 were not affected by bupranolol and CGP 20712A. 4. In conclusion, activation of the low-affinity state of β 1-adrenoceptors by CGP 12177 and cyanopindolol in pithed rats causes a positive inotropic and lusitropic effect. By contrast, the vascular effects of CGP 12177 and cyanopindolol are not mediated by these receptors and have only marginal influence under in vivo conditions. © 2005 Nature Publishing Group All rights reserved.