Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1(AP(r) lac cts62): Construction of his-lac gene fusions

Abstract

The bacteriophage Mu d1(Ap(r) lac cts62) obtained from an Escherichia coli double lysogen carrying the defective Mu d1 phage and a Mu-P1 hybrid phage was utilized as a vector for phage mutagenesis in Erwinia carotovora subsp. carotovora. Among ampicillin-resistant transductants, 1.4% were auxotrophs. The synthesis of β-galactosidase was derepressed upon starvation for histidine in two different his-lac fusion strains.