Gold Nanoparticles: Their Application as i Antimicrobial Agents and Vehicles of Gene Delivery

Abstract

Background: silver nanoparticles (AgNPs) have attracted significant interest in the field of biomedical applications and there are many methods for AgNPs synthesis. Biosynthesis of AgNPs is the most common method in in the last period. Current study aimed to determine effect of different biosynthesis methods for AgNPs on their antibacterial activity. Methods: AgNPs biosynthesis by three methods include; extract of white rot fungal strain Ganoderma enigmaticum (A), leaves of corn plant Zea mays (B) and root of Rhazya stricta plant (D). Characteristics of biosynthesis AgNPs are detected by UV-Vis spectrophotometry (UV-Vis), Field emission scanning electron microscope (FE-SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Antibacterial activity of biosynthesized AgNPs tested against five isolates of Escherichia coli (E. coil) and 5 isolates of staphylococcus aureus (S. aureus) isolated from patients diagnosed with urinary tract infection (UTI) by disk diffusion method. Results: AgNPs biosynthesis by plant extracts have the best inhibition effect on bacteria isolates in compared with fungal extract when AgNPs biosynthesized by Corn plant (B) have the highest antibacterial effect against E. coil and S. aureus (19.55±1.95 and 25.33±2.15 respectively) followed by AgNPs biosynthesis by Rhazya stricta plant (D) (15.71±1.21 and15.61±0.43 respectively). In compared with E. coil, S. aureus more effected by most antibacterial agents that used in this study but statically significant appeared toward B only (p=0.047). Conclusion: activity of biosynthesis AgNPs different according to materials which used for producing AgNPs in biosynthesis method.