A piezoelectric immunosensor for early cervical cancer detection

Abstract

Background: A piezoelectric immunosensor for early cervical cancer detection was developed involving short analyis time and less invasive technique for p16INK4a, a protein that has been linked to cervical cancer. Materials and Methods: 5μL of 5.0 mg/mL p16INK4a antibody and then supernatant from different clinical samples from West China Second University Hospital (Sichuan, China) were dripped on the center of the AT-cut crystal through a micro-injector. Absorption of the p16INK4a by antibody caused a shift in the resonant frequency of the immunosensor, and the resonant frequency was correlated to the amount of the p16INK4a in the supernatant. Results: The greater severity of lesion grading, the greater the expression level of p16INK4. Conclusion: Degree of cervical cancer lesion development could be determined by detected amount of p16INK4a in different clinical samples.