Biological effects of chicken type III interferon on expression of interferon-stimulated genes in chickens: Comparison with type I and Type II interferons

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Abstract

Interferons (IFNs) are key mediators that activate host defense mechanisms against viruses. The recently identified mammalian Type III IFN has biological effects similar to type I IFN. However, the biological effects of type III IFN have not yet been characterized in birds. We compared the effects of chicken type III IFN (IFN-λ) with type I (IFN-β) and type II (IFN-γ) IFNs on IFN-stimulated genes (ISGs) using recombinant proteins expressed in Escherichia coli. Recombinant chicken IFN-λ inhibited influenza virus replication and induced the mRNA expression of the ISGs, Mx and OAS, in chicken embryonic fibroblasts (CEFs) in a dose-dependent manner. However, the effective dose of IFN-λ was higher than that of IFN-β and IFN-γ. Furthermore, the effect of IFN-λ on induction of Mx and OAS was lesser than that of IFN-β, but comparable to that of IFN-γ. These results indicate that chicken IFN-λ has the potential to induce ISGs and inhibit viral replication in chicken cells. © 2012 The Japanese Society of Veterinary Science.

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Masuda, Y., Matsuda, A., Usui, T., Sugai, T., Asano, A., & Yamano, Y. (2012). Biological effects of chicken type III interferon on expression of interferon-stimulated genes in chickens: Comparison with type I and Type II interferons. Journal of Veterinary Medical Science, 74(11), 1381–1386. https://doi.org/10.1292/jvms.11-0517

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