Antioxidant Activity of Collagen from the Skin of Pangas Catfish (Pangasius sp) with Crude Bromelain Enzyme of Pineapple Peel

Abstract

Pangas catfish skins is one of the fishery by-products containing certain compounds which have the potential to be used as an alternative source of collagen having antioxidant activity. The purpose of this study is to determine the antioxidant activity of collagen from the skin of pangas catfish that extracted with bromelain enzyme of pineapple peel. The collagen was made using crude bromelain enzyme from pineapple peel with an activity of 1.0 U/g in several stages, which were pretreatment, deproteinization, degreasing, and extraction, using data analysis a combination of 3; 3.5; and 4 hours with crude enzyme concentrations of 0; 1.5%; 2%. The data was analysed using completely randomized design (CRD). The results showed that the best solubility was obtained in the T3.E2 (4 hours, concenration 1.5%) treatment which was 98.96%. The best viscosity was 22.67 cP. The best acidity (pH) at T1.E2 (3 hours, concentration 1.5%) was 6.73. The molecular weights α1, α2, β and ƴ were: 131.51 kDa, 110.48 kDa, 202.48 kDa and 243.93 kDa. The best antioxidant activity was in the T1.E3 (3 hours, concentration 2%) treatment, which was 20.45 ferrous sulfate/g.