Identification and quantification of β- sitosterol in leaf of calotropis gigantea and calotropis procera by HPTLC

Abstract

Objective: The distinguishing proof and measurement of biomarker β-sitosterol present in a leaf of Calotropis procera and Calotropis gigantea belonging to family Asclepidiaceae was carried by utilizing high performance thin-layer chromatographic (HPTLC) characterization method. In the present research work the Exterinsic Standard Method is used for quantitative assessment. Material and Method: It assures the accuracy of quantitative investigation and particularly benifit for HPTLC analysis. Aluminum-maintained silica gel 60F254 HPTLC plates of 250μm thickness was used for chromatographic analysis. Toluene: Ethyl acetate: Glacial acetic acid acidic 8:2:0.20 (v/v/v) solvent system was used as mobile phase. Anisaldehyde sulphuric acid is used for derivatisation and was evaluated at 510 nm. Result and Discussion: β-sitosterol quantification in foundations of plant was observed as 11.63 µg and 10.23 µg for Calotropi sprocera and Calotropis gigantea respectively. The appropriateness and particularity of the established techniques was demonstrated by qualitative and quantative analysis. This established method for estimation of β-sitosterol in the leaf ofCalotropis procera and Calotropis gigantea was proved to be responsive, particular and replicable.