The Development of Diagnostics Tools and Techniques in the Isolation and Detection of Fungal Pathogens

Abstract

Until of the recent, the use of molecular tools in diagnostic mycology laboratories supersedes the old conventional methods. The diagnostics laboratories consider two important parameters; sensitivity and specificity, and the use of PCR and it is related have it all. This review went through the timeline of how fungal pathogen detection techniques have developed and still in the progress. The most recent other review articles and research papers were selected at random and critical analyses are made on the previous studies and papers. It is ascertain that molecular tools are more sensitive and specific and hence accurate results are achieved. It is clear that even though molecular techniques are reliable enough, the methods and the purity of DNA extraction and the amount respectively determines the fingerprint that gives identity to the respective pathogens.