Method development and validation for determination of voglibose in tablet formulation using LC-MS/MS

Abstract

Voglibose is a potent a glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed and validated. The method was found linear in the concentration range of 25.0-1200 hg/mL with a correlation coefficient of 0.9998. The limit of detection (LOD) of the method was found to be 1.5 hg/mL and limit of quantitation (LOQ) was achieved at 3.0 hg/mL. The recoveries of voglibose from spiked samples at different concentration levels were found in the range of 98-102%. The proposed method was found suitable for quantitation of voglibose and for the determination of uniformity of content of the dosage units of the tablet formulations. © Copyright E-Journal of Chemistry 2004-2011.