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High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Applied and Environmental Microbiology (2012) 78(13) 4740-4743
DOI: 10.1128/AEM.00069-12
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Abstract

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening. © 2012, American Society for Microbiology.

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Lee, S. F., White, V. L., Weeks, A. R., Hoffmann, A. A., & Endersby, N. M. (2012). High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans. Applied and Environmental Microbiology, 78(13), 4740–4743. https://doi.org/10.1128/AEM.00069-12

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