Isolation and Molecular Identification of Colletotrichum gloeosporioides from Infected Peanut Seeds

Abstract

Peanut (Arachis hypogaea (L) cv. Margenta) seeds are currently, significantly considered as an important source of protein and vegetable oil. Anthracnose caused by Colletotrichum gloeosporioides is a fungal disease from seed that reduces seedling emergence and seed germination. The pathogenic fungi that isolated from peanut were identified using ITS 1 and ITS 4 primer with polymerase chain reaction and NCBI database using BlastN algorithm. From NCBI using blastN analysed was 95% identify with other Colletotrichum species in NCBI database search. The pathogenicity of Colletotrichum gloeosporioides on peanut seeds and seedlings at 14 days after sowing result was inoculated with pathogenic fungi with 32.3% and control was 83%. Hence, this study was experimented with the object to reveal seed-borne infections on tropical peanut in Malaysia.