Geometric morphometrics as a tool for understanding Dactylorhiza (Orchidaceae) diversity in European Russia

Abstract

Geometric morphometric techniques were employed to assess the diversity of lip shapes (305 samples from 83 populations) in flowers of European Russian Dactylorhiza (Orchidaceae: Orchidinae). We found significant agreement between the results from geometric morphometrics, classic morphometrics and the distribution of certain nuclear DNA markers. The lip shapes from Arctic Dactylorhiza samples occupied an intermediate position between D. maculata and D. fuchsii samples from Central Russia, supporting a hybrid origin of 'northern tetraploids'. Lip shapes of the taxonomically controversial allotetraploid D. baltica were found to form a distinct group, with members having definite relationships with diploid D. incarnata samples from the same localities, indicating either their local origin or introgression with D. incarnata. In addition to demonstrating the value of geometric morphometric methods in studies of plant taxonomy and hybridization, we suggest future applications designed to explore pollinator-driven directional selection, developmental constraints and fluctuating asymmetry. © 2005 The Linnean Society of London.