Near-infrared Spectroscopy Applications in the Quantitative Determination of Bovine Genomic DNA Content from Milk

Abstract

This study was to provide a convenient method for the determination of DNA concentration from milk. Near infrared (NIR) spectroscopy technology with orthogonal experiment method was conducted to determine bovine genomic DNA content in milk for the first time. DNA was extracted from milk samples with a novel method that was constructed based on our pre-experiments results. All samples were scanned (570-1850 nm), and 74 samples were used to establish the model of DNA content in NIR analysis. The results showed that the optimal model's performance for DNA content was excellent, with SEC = 0.26%, SECV = 0. 69%, SEP = 0. 28%, RSQ = 0.913 and R 2 = 0.9846, RPD = 3.57 respectively. Thus NIR was feasible to nondestructively determine DNA content in milk. Therefore, NIR technology would be valuable in meeting with the increasing demand for monitoring the dairy food chain and ensuring labeling authenticity to the consumers.