Localization of dystrophin relative to acetylcholine receptor domains in electric tissue and adult and cultured skeletal muscle

117Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.

Abstract

Two high-affinity mAbs were prepared against Torpedo dystrophin, an electric organ protein that is closely similar to human dystrophin, the gene product of the Duchenne muscular dystrophy locus. The antibodies were used to localize dystrophin relative to acetylcholine receptors (AChR) in electric organ and in skeletal muscle, and to show identity between Torpedo dystrophin and the previously described 270/300-kD Torpedo postsynaptic protein. Dystrophin was found in both AChR-rich and AChR-poor regions of the innervated face of the electroplaque. Immunogold experiments showed that AChR and dystrophin were closely intermingled in the AChR domains. In contrast, dystrophin appeared to be absent from many or all AChR-rich domains of the rat neuromuscular junction and of AChR clusters in cultured muscle (Xenopus laevis). It was present, however, in the immediately surrounding membrane (deep regions of the junctional folds, membrane domains interdigitating with and surrounding AChR domains within clusters). These results suggest that dystrophin may have a role in organization of AChR in electric tissue. Dystrophin is not, however, an obligatory component of AChR domains in muscle and, at the neuromuscular junction, its roles may be more related to organization of the junctional folds.

Cite

CITATION STYLE

APA

Sealock, R., Butler, M. H., Kramarcy, N. R., Gao, K. X., Murnane, A. A., Douville, K., & Froehner, S. C. (1991). Localization of dystrophin relative to acetylcholine receptor domains in electric tissue and adult and cultured skeletal muscle. Journal of Cell Biology, 113(5), 1133–1144. https://doi.org/10.1083/jcb.113.5.1133

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free