On the Activation of Fructose‐1,6‐bisphosphatase of Spinach Chloroplasts and the Regulation of the Calvin Cycle

Abstract

Activation by light of spinach fructose‐1,6‐bisphosphatase is mimicked by dithiothreitol. This process of activation by dithiothreitol implies the specific reduction of two disulfide bridges and a conformation change of the enzyme that makes eight sulfhydryl groups available. The activated enzyme has an apparent allosteric kinetic behavior with respect to both fructose‐1,6‐bisphosphate and magnesium. Copyright © 1981, Wiley Blackwell. All rights reserved