Keragaman Sekuen Gen Reseptor Hormon Pertumbuhan Exon 10 sebagai Informasi Dasar Seleksi pada Sapi Pesisir Plasma Nutfah Sumatera Barat

Abstract

From a total of 216 of 1,5 year old Pesisir cattle,60 were selected on the basis of weight, were 30 with highest weight (125±9 kg) and 30 with lower weight (65±6 kg). The sample were genotyped for growth hormone receptor (GHR) gene by sekuensing (SNP) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Five deletion were detected in position 34, 39, 45, 50, and 115 with frequency allele were 0.45, 0.15, 0.46, 0.46, and 1.00 respectively and 4 insertions were detected in position 67, 74, 113, and 133 with frequency allele were 0,19, 0.19, 0.25, and 0.45 respectively with the genotypes insertion T, A , G, and G. Four mutation were detected in position 32, 50, 109, and 150 with genotypes T ? C, G ? A, A ? C, and G? C respectively with frequency allele were 0.50, 0.23, 0.19, and 1,00 respectively. The GHRNlaIII gene frequencies for the T and C allele were 0.017 and 0.983 respectively. The chi- square analysis indicated that this population was not in Hardy-Weinberg Equilibrium status. This finding showed that GHRNlaIII was not favorable as a GHR marker for Bos indicus breeds. There was no relationship beetwen variability and two group of cattle with these marker. These data provide evidence that GHR gene was good polymorphic source and can be used for association with performance and investigate whether this polymorphic might be responsible for quantitative variation in growth.