Kinetic Study on Decolorization of the Dye Acid Orange Using the Fungus Phanerochate Chrysosporium

Abstract

A dye decolorizing fungal strain, Phanerochaete chrysosporium MTC 787 was used in batch experiments for decolorization of the dye, Acid orange10. Effects of initial substrate (dye) and fungal concentrations on the rate and extent of dye decolorization were investigated. The initial pH and oxidation-reduction potential (ORP) was kept at 4.5 and −250 mV, respectively. The rate and extent of dye decolorization increased with increasing dye concentration up to 100 g l −1 and then decreased for larger dye concentrations. The rate of enzyme utilization and dye decolorization also increased linearly with increasing initial biomass concentration. A kinetic model describing the rate of enzyme utilization and substrate inhibition as function of the initial substrate and flow rate was developed. The kinetic constants were determined using the experimental data. The initial biomass should be above 3.2 x 10 5 cell/ml to obtain high rates and decolorization percentage and to avoid substrate inhibition.