Evaluation of Salivary Interleukin 6 and MicroRNA 146a in Patients with Chronic Periodontitis and Their Association with Periodontal Parameters

Abstract

Objective: The aim of this study was to evaluate of the role of interleukin 6 (IL-6) and microRNA 146a (miRNA 146a) in chronic periodon, s (CPD). Patients and Methods: This cross-sec, onal case control study was conducted at the Diyala province-Iraq. A total of 40 CPD pa, ents were included. They were 20 males and 20 females, and their age range was 23-56 years. The clinical periodontal parameters were measured under supervision of specialized den, sts. Furthermore, 40 apparently healthy individuals, 21 were males and 19 were female, were included as controls. Their age range was 20-50 years. All par, cipants were requested to complete a ques, onnaire that contained medical and socio-demographic data. The salivary IL-6 was determined using qELISA (INOVA Biotech Co., LTD, China). The salivary miRNA 146a was detected using qPCR (TransGen biotech, China). Sta, s, cal analyses were done using SPSS version 22 (Inc., Chicago, Illinois, United States), and P-values ≤ 0.05 were considered significant. Results: The mean age ± SD of pa, ents was not different than that of controls (38.85 ± 5.30 Vs 40.52 ± 6.69, P=0.2). The gender was also not different (P=0.8). The mean ± SD PI of CPD pa, ents was significantly higher (1.27± 0.34 Vs 0.60 ± 0.26, P=0.01). Similarly, the mean ± SD of GI was also significantly higher in CPD pa, ents (1.49 ± 0.42 Vs 0.24 ± 0.14, P= 0.01). The rate of BOP sites in CPD pa, ents was significantly higher than that of controls (P=0.001). Also, the mean ± SD of PPD in CPD pa, ents was 4.66 ± 0.50 mm, while the mean ± SD of CAL was 4.90 ± 1.30. Addi, onally, the mean No.± SD of teeth in CPD pa, ents was significantly lower (24.72± 3.77 Vs 27.62± 0.80, P=0.01). Furthermore, the mean No. ± SD of sites in CPD pa, ents was also significantly lower (98.95± 15.08 Vs 110.50 ± 3.22, P 0.01). The mean concentra, on ± SD of salivary IL-6 was not higher in CPD pa, ents (8.17 ± 3.79 Vs 7.45± 1.51, P= 0.4). qPCR results found that there was significantly higher expression of miRNA-146a in CPD pa, ents versus that of the controls (1.79 ± 0.10 Vs 1.08± 0.10, P=0.001). Conclusion: The current results concluded that both IL-6 and miRNA 146a were elevated in CPD pa, ents and closely correlated with periodontal parameters sugges, ng that these biomarkers can be used as a surrogate diagnos, c and prognos, c indicator of chronic periodon, s.