Effect of pup out® on caspase 3, and interleukin-1expression in pulp teeth: A paste contained jatropha, sidaguri, and melittin

Abstract

Objective: To examine the expression of caspase-3 and IL-1β following application of Pulp Out® on rabbit’s pulp teeth as well as histopathology. Material and Methods: The study was conducted on the maxillary incisors rabbits. The teeth were prepared and Pulp Out® was inserted at the base of prepared cavity, then restored with RM-GIC. After 24 hours, the animals were then euthanized and processed for histopathology and immunohistochemistry evaluation. Results: Our results indicated that Pulp Out®, when administered to the pulp teeth, increased the number and diameter of blood vessels. The expression of caspase-3 and IL-1β showed similarities to the expression of commercial devitalizing agent. Conclusion: Our results revealed that Pulp Out® is a valuable devitalization agent that should be further explored for its safety before clinical application.