In Vitro Effect of Boron Compounds in Combination with Photobiomodulation Therapy by 905 nm on the Viability of Human Gingival Fibroblasts

Abstract

This study aimed to evaluate the effect of Low-level laser therapy (LLLT) and Boric acid (BA) or Borax decahydrate (BD) on the cell survival of gingival fibroblasts. Fibroblast planted plates were divided into 10 groups: Control group, BD 100 µg/mL (BD 100), BD 200 µg/mL (BD200), BA 100 µg/mL (BA100), BA 200 µg/mL (BA200), LLLT, LLLT+BD100 µg/mL (LLLT+BD100), LLLT+BD200 µg/mL (LLLT+BD200), LLLT+BA100 µg/mL (LLLT+BA100) and LLLT+BA 200 µg/mL (LLLT+BA200) groups. LLLT was performed at a dose of 4 J/cm2 for 160 sec. Following LLLT, MTT analysis was performed after the cells were kept in the incubator for 24 h. The LLLT+BA 200 group exhibited the highest cell density. In MTT analysis, significantly higher cell numbers were observed in the BA200, LLLT+BA100, and LLLT+BA200 groups compared to the control group (P<0.05). Then, the 3-(4,5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide assay and 8-hydroxy-2’-deoxyguanosine and Bcl-2 Associated X-Protein were analyzed. The 8-hydroxy-2’-deoxyguanosine and Bcl-2 Associated X-Protein levels were decreased in the LLLT+BA200 group compared to the control group (P<0.05). This study suggests that BA200, LLLT+BA100, and LLLT+BA200 increase the survival of fibroblast cells.